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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo01.raw Sample ID: test oligo 01 Position: 1 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 cttcCATCACCACCCCAC Termini: H, OH Average Mass (Da): 5348.7 Monoisotopic Mass (Da): 5345.8 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.25 | 5348.7 | 5348.3 | -0.4 Da (-0.007 %) | 5.00E+005 | 64.3 | Target Mass | P |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.25 | 5348.3 | 5.00E+005 | ok | 1.50E+007 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 01:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 01:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 01, RT = 0.25 min:
Scan Mode: - c Full ms
Scans Averaged: 7-9 Minus: 3-4, 14-15
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[ESI Mass Spectrum]
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[Deconvolution Peak Report]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 01, RT = 0.25 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
[View Data]
[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 01, RT = 0.25 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 5348.3 | 5.00E+005 | 0.0 | 100.0 | 64.3 | Target Mass: 5348.7 |
| 5213.1 | 1.99E+005 | -135.2 | 39.9 | 25.6 | 5348.7 (A depurination) |
| 5236.8 | 2.37E+004 | -111.5 | 4.7 | 3.1 | 5348.7 (C depyrimidination) |
| 6529.2 | 1.18E+004 | 1180.9 | 2.4 | 1.5 | ? |
| 2318.8 | 1.15E+004 | -3029.5 | 2.3 | 1.5 | ? |
| 5385.7 | 1.09E+004 | 37.4 | 2.2 | 1.4 | 5348.7 (*K adduct) |
| 5034.4 | 9.16E+003 | -313.9 | 1.8 | 1.2 | 5348.7 (Minus A) |
| 2971.3 | 6.23E+003 | -2377.0 | 1.2 | 0.8 | ? |
| 3272.9 | 5.09E+003 | -2075.4 | 1.0 | 0.7 | ? |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |