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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo02.raw Sample ID: test oligo 02 Position: 2 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 cggcACCTAAGCACTGGG Termini: H, OH Average Mass (Da): 5573.9 Monoisotopic Mass (Da): 5570.9 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.32 | 5573.9 | 5572.1 | -1.8 Da (-0.032 %) | 2.24E+005 | 65.0 | Target Mass | G |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.32 | 5572.1 | 2.24E+005 | ok | 8.80E+006 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 02:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 02:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 02, RT = 0.32 min:
Scan Mode: - c Full ms
Scans Averaged: 9-11 Minus: 5-6, 16-17
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[ESI Mass Spectrum]
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[Deconvolution Peak Report]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 02, RT = 0.32 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
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[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 02, RT = 0.32 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 5572.1 | 2.24E+005 | 0.0 | 100.0 | 65.0 | Target Mass: 5573.9 |
| 5438.8 | 2.51E+004 | -133.3 | 11.2 | 7.3 | 5573.9 (A depurination) |
| 5462.5 | 1.76E+004 | -109.6 | 7.9 | 5.1 | 5573.9 (C depyrimidination) |
| 6506.8 | 1.50E+004 | 934.7 | 6.7 | 4.4 | ? |
| 6373.0 | 1.24E+004 | 800.9 | 5.5 | 3.6 | ? |
| 5595.1 | 8.73E+003 | 23.0 | 3.9 | 2.5 | 5573.9 (*Na adduct) |
| 4369.3 | 7.64E+003 | -1202.8 | 3.4 | 2.2 | ? |
| 4677.7 | 4.91E+003 | -894.4 | 2.2 | 1.4 | ? |
| 5285.3 | 3.85E+003 | -286.8 | 1.7 | 1.1 | 5573.9 (Minus C) |
| 5421.6 | 3.56E+003 | -150.5 | 1.6 | 1.0 | 5573.9 (G depurination) |
| 4274.7 | 3.55E+003 | -1297.4 | 1.6 | 1.0 | ? |
| 2746.9 | 3.02E+003 | -2825.2 | 1.4 | 0.9 | ? |
| 3030.8 | 2.71E+003 | -2541.3 | 1.2 | 0.8 | ? |
| 3108.4 | 2.69E+003 | -2463.7 | 1.2 | 0.8 | ? |
| 3505.4 | 2.61E+003 | -2066.7 | 1.2 | 0.8 | ? |
| 3817.8 | 2.51E+003 | -1754.3 | 1.1 | 0.7 | ? |
| 3710.6 | 2.41E+003 | -1861.5 | 1.1 | 0.7 | ? |
| 4185.5 | 2.25E+003 | -1386.6 | 1.0 | 0.7 | ? |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |