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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo03.raw Sample ID: test oligo 03 Position: 3 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 CCGGGGTGACCACACAGC Termini: H, OH Average Mass (Da): 5494.6 Monoisotopic Mass (Da): 5492.0 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.32 | 5494.6 | 5494.5 | -0.1 Da (-0.002 %) | 4.08E+005 | 53.2 | Target Mass | G |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.32 | 5494.5 | 4.08E+005 | ok | 1.63E+007 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 03:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 03:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 03, RT = 0.32 min:
Scan Mode: - c Full ms
Scans Averaged: 9-11 Minus: 5-6, 16-17
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[ESI Mass Spectrum]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 03, RT = 0.32 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
[View Data]
[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 03, RT = 0.32 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 5494.5 | 4.08E+005 | 0.0 | 100.0 | 53.2 | Target Mass: 5494.6 |
| 5204.9 | 8.60E+004 | -289.6 | 21.1 | 11.2 | 5494.6 (Minus C) |
| 5180.4 | 4.74E+004 | -314.1 | 11.6 | 6.2 | 5494.6 (Minus A) |
| 4555.0 | 4.33E+004 | -939.5 | 10.6 | 5.7 | ? |
| 5360.0 | 3.73E+004 | -134.5 | 9.1 | 4.9 | 5494.6 (A depurination) |
| 4603.0 | 2.43E+004 | -891.5 | 6.0 | 3.2 | ? |
| 6186.4 | 2.25E+004 | 691.9 | 5.5 | 2.9 | ? |
| 6471.9 | 1.28E+004 | 977.4 | 3.1 | 1.7 | ? |
| 3381.0 | 1.24E+004 | -2113.5 | 3.0 | 1.6 | ? |
| 6922.0 | 1.05E+004 | 1427.5 | 2.6 | 1.4 | ? |
| 5529.1 | 1.03E+004 | 34.6 | 2.5 | 1.3 | ? |
| 5903.0 | 9.73E+003 | 408.5 | 2.4 | 1.3 | ? |
| 2778.9 | 8.01E+003 | -2715.6 | 2.0 | 1.0 | ? |
| 5548.1 | 7.65E+003 | 53.6 | 1.9 | 1.0 | 5494.6 (Plus DMF) |
| 5343.3 | 6.40E+003 | -151.2 | 1.6 | 0.8 | 5494.6 (G depurination) |
| 6887.0 | 5.56E+003 | 1392.5 | 1.4 | 0.7 | ? |
| 3093.1 | 5.44E+003 | -2401.4 | 1.3 | 0.7 | ? |
| 5918.9 | 5.23E+003 | 424.4 | 1.3 | 0.7 | ? |
| 5382.6 | 4.12E+003 | -111.9 | 1.0 | 0.5 | 5494.6 (C depyrimidination) |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |