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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo04.raw Sample ID: test oligo 04 Position: 4 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 AGGCCTTCCCTCCGCCTG Termini: H, OH Average Mass (Da): 5387.5 Monoisotopic Mass (Da): 5384.9 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.32 | 5387.5 | 5387.2 | -0.3 Da (-0.006 %) | 3.92E+005 | 77.6 | Target Mass | G |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.32 | 5387.2 | 3.92E+005 | ok | 9.26E+006 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 04:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 04:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 04, RT = 0.32 min:
Scan Mode: - c Full ms
Scans Averaged: 9-11 Minus: 5-6, 16-17
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[ESI Mass Spectrum]
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[Deconvolution Peak Report]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 04, RT = 0.32 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
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[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 04, RT = 0.32 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 5387.2 | 3.92E+005 | 0.0 | 100.0 | 77.6 | Target Mass: 5387.5 |
| 5275.2 | 2.83E+004 | -112.0 | 7.2 | 5.6 | 5387.5 (C depyrimidination) |
| 5073.3 | 1.98E+004 | -313.9 | 5.1 | 3.9 | 5387.5 (Minus A) |
| 5154.4 | 1.14E+004 | -232.8 | 2.9 | 2.3 | ? |
| 6283.3 | 9.71E+003 | 896.1 | 2.5 | 1.9 | ? |
| 6327.7 | 9.67E+003 | 940.5 | 2.5 | 1.9 | ? |
| 5425.3 | 8.86E+003 | 38.1 | 2.3 | 1.8 | 5387.5 (*K adduct) |
| 2192.1 | 6.53E+003 | -3195.1 | 1.7 | 1.3 | ? |
| 4963.3 | 5.35E+003 | -423.9 | 1.4 | 1.1 | ? |
| 3942.2 | 5.00E+003 | -1445.0 | 1.3 | 1.0 | ? |
| 4824.4 | 4.25E+003 | -562.8 | 1.1 | 0.8 | ? |
| 3702.2 | 4.12E+003 | -1685.0 | 1.1 | 0.8 | ? |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |