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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo06.raw Sample ID: test oligo 06 Position: 6 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 aaagCAAACTCATTGCTCCTCGTA Termini: H, OH Average Mass (Da): 7345.0 Monoisotopic Mass (Da): 7341.2 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.32 | 7345.0 | 7344.4 | -0.6 Da (-0.008 %) | 5.54E+005 | 59.0 | Target Mass | G |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.32 | 7344.4 | 5.54E+005 | ok | 2.16E+007 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 06:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 06:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 06, RT = 0.32 min:
Scan Mode: - c Full ms
Scans Averaged: 9-11 Minus: 5-6, 16-17
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[ESI Mass Spectrum]
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[Deconvolution Peak Report]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 06, RT = 0.32 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
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[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 06, RT = 0.32 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 7344.4 | 5.54E+005 | 0.0 | 100.0 | 59.0 | Target Mass: 7345.0 |
| 7208.9 | 1.62E+005 | -135.5 | 29.2 | 17.2 | 7345.0 (A depurination) |
| 7031.5 | 1.12E+005 | -312.9 | 20.2 | 11.9 | 7345.0 (Minus A) |
| 6895.5 | 2.23E+004 | -448.9 | 4.0 | 2.4 | ? |
| 5777.9 | 1.94E+004 | -1566.5 | 3.5 | 2.1 | ? |
| 7381.4 | 1.81E+004 | 37.0 | 3.3 | 1.9 | 7345.0 (*K adduct) |
| 6358.7 | 1.53E+004 | -985.7 | 2.8 | 1.6 | ? |
| 7233.7 | 1.38E+004 | -110.7 | 2.5 | 1.5 | 7345.0 (C depyrimidination) |
| 6451.6 | 1.05E+004 | -892.8 | 1.9 | 1.1 | ? |
| 2666.1 | 6.22E+003 | -4678.3 | 1.1 | 0.7 | ? |
| 7072.6 | 5.69E+003 | -271.8 | 1.0 | 0.6 | ? |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |