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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo10.raw Sample ID: test oligo 10 Position: 10 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 catgCAGGCAAAAGCGGC Termini: H, OH Average Mass (Da): 5606.9 Monoisotopic Mass (Da): 5603.9 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.32 | 5606.9 | 5590.5 | -16.4 Da (-0.292 %) | 6.70E+005 | 76.4 | 5606.9 (loss of thioate) | R |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.32 | 5590.5 | 6.70E+005 | ok | 1.63E+007 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 10:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 10:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 10, RT = 0.32 min:
Scan Mode: - c Full ms
Scans Averaged: 9-11 Minus: 5-6, 16-17
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[ESI Mass Spectrum]
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[Deconvolution Peak Report]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 10, RT = 0.32 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
[View Data]
[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 10, RT = 0.32 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 5590.5 | 6.70E+005 | 0.0 | 100.0 | 76.4 | 5606.9 (loss of thioate) |
| 5455.2 | 1.42E+005 | -135.3 | 21.2 | 16.2 | 5606.9 (G depurination) |
| 5628.0 | 1.79E+004 | 37.5 | 2.7 | 2.0 | 5606.9 (*Na adduct) |
| 4307.4 | 1.71E+004 | -1283.1 | 2.6 | 2.0 | ? |
| 5261.4 | 1.54E+004 | -329.1 | 2.3 | 1.8 | 5606.9 (Minus g) |
| 6276.3 | 7.35E+003 | 685.8 | 1.1 | 0.8 | ? |
| 1857.8 | 6.91E+003 | -3732.7 | 1.0 | 0.8 | ? |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |