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Data File: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\oligo14.raw Sample ID: test oligo 14 Position: 14 Processing Method: C:\Program Files\ProMassXcali\ProMass Example Data\HT_oligo\HT_oligo Nucleotide: 1--------10--------20--------30--------40--------50 AACTGGAAAAGGTGAGTATZGTAACCC Termini: H, OH Average Mass (Da): 8190.4 Monoisotopic Mass (Da): 8186.4 |
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| RT (min) | Target Mass (Da) | Observed Mass (Da) | Mass Error | Intensity | % Total Abundance |
Identity | Result Code |
|---|---|---|---|---|---|---|---|
| 0.32 | 8190.4 | 8189.9 | -0.5 Da (-0.006 %) | 4.68E+005 | 55.2 | Target Mass | P |
| RT (min) | Base Peak Mass (Da) | Intensity | Spectral Quality | LC/MS Peak Area | LC/MS Area Percent |
|---|---|---|---|---|---|
| 0.32 | 8189.9 | 4.68E+005 | ok | 1.93E+007 | 100.00 |
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[LC/UV 260 nm]
LC/MS Chromatogram of test oligo 14:
TIC
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[LC/MS]
LC/UV 260 nm Chromatogram of test oligo 14:
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[Deconvolution]
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[Deconvolution Peak Report]
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[Log File]
ESI Mass Spectrum of test oligo 14, RT = 0.32 min:
Scan Mode: - c Full ms
Scans Averaged: 9-11 Minus: 5-6, 16-17
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[ESI Mass Spectrum]
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[Log File]
Deconvoluted Mass Spectrum of test oligo 14, RT = 0.32 min:
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[ESI Mass Spectrum]
[Deconvolution]
[Deconvolution Peak Report]
[View Data]
[Log File]
Zoom Deconvoluted Mass Spectrum of test oligo 14, RT = 0.32 min:
| Mass (Da) | Intensity | Delta Mass |
%Relative | %Total | Presumed Identity |
|---|---|---|---|---|---|
| 8189.9 | 4.68E+005 | 0.0 | 100.0 | 55.2 | Target Mass: 8190.4 |
| 8054.4 | 1.57E+005 | -135.5 | 33.4 | 18.4 | 8190.4 (Minus Z), or 8190.4 (A depurination) |
| 8228.1 | 3.75E+004 | 38.2 | 8.0 | 4.4 | 8190.4 (*K adduct) |
| 7876.8 | 3.16E+004 | -313.1 | 6.7 | 3.7 | 8190.4 (Minus A) |
| 4083.0 | 2.54E+004 | -4106.9 | 5.4 | 3.0 | ? |
| 5322.7 | 1.35E+004 | -2867.2 | 2.9 | 1.6 | ? |
| 4912.5 | 1.18E+004 | -3277.4 | 2.5 | 1.4 | ? |
| 4049.3 | 1.02E+004 | -4140.6 | 2.2 | 1.2 | ? |
| 3494.1 | 9.97E+003 | -4695.8 | 2.1 | 1.2 | ? |
| 9491.5 | 8.98E+003 | 1301.6 | 1.9 | 1.1 | ? |
| 7921.3 | 8.93E+003 | -268.6 | 1.9 | 1.1 | ? |
| 7515.2 | 8.69E+003 | -674.7 | 1.9 | 1.0 | ? |
| 7564.1 | 7.73E+003 | -625.8 | 1.7 | 0.9 | ? |
| 5073.8 | 7.50E+003 | -3116.1 | 1.6 | 0.9 | ? |
| 8879.7 | 7.20E+003 | 689.8 | 1.5 | 0.8 | ? |
| 2740.9 | 6.87E+003 | -5449.0 | 1.5 | 0.8 | ? |
| 8101.3 | 6.61E+003 | -88.6 | 1.4 | 0.8 | ? |
| 7535.6 | 6.10E+003 | -654.3 | 1.3 | 0.7 | ? |
| 3663.4 | 5.77E+003 | -4526.5 | 1.2 | 0.7 | ? |
| 1792.1 | 5.03E+003 | -6397.8 | 1.1 | 0.6 | ? |
| 7266.5 | 4.92E+003 | -923.4 | 1.1 | 0.6 | ? |
| Result Code | Indication |
|---|---|
| Target mass found in chromatogram as the most abundant component within 0.02% mass error tolerance | |
| Target mass found as a major component or as a minor component with other target masses, but NOT as most abundant component in chromatogram | |
| Target mass found in chromatogram with either or all of the following: (a) other non-target components present in spectrum > 30% relative abundance (b) low spectral quality (low intensity and/or score) | |
| Target mass found in chromatogram, but NOT as the most abundant in any of the chromatographic peaks | |
| Target mass NOT found in chromatogram within 0.02% mass error tolerance | |
| No target masses specified |