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Example of high-throughput biomolecule-LC/MS system
Sample Analysis Using Trap/Wash/Elute Scheme
waste
manual
inject
waste
LC flow
in
Cap trap
manual inj
loop
1
10
2
4
3
5
6
7
8
9
needle
sample
loop
Wash
solvent
priming valve
priming
syringe
10
2
3
4
5
6
7
8
9
Michrom mHPLC
96 well autosampler
UV
0.5 mm i.d.
column
50 mL/min
TSQ7000
finnigan
Reset me!
ESI/MS/MS
system
Key Components
•Automated instrument control of trap/wash/elute process •Large pore short polymeric columns 0.5x50mm PLRP-4000
•4X normal flow rate 50uL/min
•low volume, high pressure mixing •limited capacity “cap trap” prevents overloading column
•3 min gradient
•post-analysis column/trap cleanup with 70%formic/30%ACN (“protein drano”)