The user sets up an Xcalibur sequence with a valid ProMass-enabled Qual
processing method and optional ZNova parameter string(s).
This overview describes how ProMass processes data automatically. If you want to manually process individual spectra, please refer to the instructions for processing a clipboard spectrum.
The user sets up an Xcalibur sequence with a valid ProMass-enabled Qual
processing method and optional ZNova parameter string(s).
The user either runs a sequence and
selects post-acquisition processing, or batch
reprocesses a sequence.
The processing method creates a
results file (.RST file) in the data file path for each data file processed.
ProMass gets chromatographic
peak definition from the results files.
ProMass averages scans that
are defined by each peak in the Xcalibur results files.
ProMass writes out an
ASCII (text) file containing sample, chromatogram, and mass - intensity
information for each spectrum averaged in the chromatogram. A ProMass ASCII data file
is created for each raw data file processed. These text files are saved in the
data file path.
ZNova reads each ProMass ASCII
data file and processes (deconvolutes) all of the averaged spectra present in
each text file.
ZNova generates graphics files,
text-based results files, and an html report for each data file processed.
ZNova produces a summary index.html
file in the results directory summarizing all of the files that were processed
into that same directory.